The Laboratory of Nano-Bioengineering, NRNU MRPhI, published two studies in the Framework of the Crystal-Bio and Plasmon-Bio projects under the Federal Target Program of the RF Ministry of Health

Two studies carried out in LNBE, funded by the Ministry of Health of the Russian Federation in the framework of the Federal Target Program "National System of Chemical and Biological Safety of the Russian Federation, 2015–2020," have been published by the Bulletin of RMSU, a biomedical journal of Pirogov Russian National Research Medical University, Moscow, Russia. Both studies present new solutions in the field of multiple pathogen detection in biological material for medical diagnostic purposes.

The theoretical study "Comparison of fluorescence excitation modes for cdse semi-conductor quantum dots used in medical research" by Y.A. Kuzishchin, I.L. Martynov, E.V. Osipov, P.S. Samokhvalov, A.A. Chistyakov, and I.R. Nabiev is the search for the optimal modes of excitation and detection of fluorescent signals from colloidal quantum dots. This issue is of practical importance because quantum dots are the best fluorescent labels to be used in systems of simultaneous detection of several biological agents in a single sample. The authors have derived a mathematical expression for estimating the signal-to-noise ratio for the excitation of fluorescence of CdSe/ZnS quantum dots in the pulsed and modulated modes. It has been found that, in medical diagnostic settings, the modulated excitation mode provides a more effective pathogen detection.

In the study "Multiparametric detection of bacterial contamination based on the photonic crystal surface mode detection" by I.O. Petrova, V.N. Konopsky, A.V. Sukhanova, and I.R. Nabiev, a new highly accurate label-free technique is suggested for multiplexed detection of pathogens. The technique relies on the analysis of surface wave propagation in photonic crystals. It allows real-time detection of the affine interaction between antigens in a solution pumped through a microfluidic cell and detector antibodies bound to the surface of a multilayered photonic crystal. To demonstrate the efficiency of the new method, the authors have developed and successfully tested protocols for detecting two bacterial toxins. The proposed technique has the advantages of highly sensitive, quick, and time-consuming analysis.

Implementation of these results into practice will result in more accurate and reliable detection of pathogens and biomarkers of infectious diseases, which is the main goal of the RF Ministry of Health's Federal Target Program "National System of Chemical and Biological Safety of the Russian Federation, 2015–2020."

The Bulletin of RMSU, published since 2016, is the English version of the Vestnik RGMU, a peer-reviewed open-access journal of the Pirogov Medical University. In its original Russian version, the journal has been published since 1994. The Bulletin of RMSU was accepted by Scopus in 2017.

Contacts:

Yuriy A. Kuzishchin, PhD, junior research assistant (yriy.kuzma@gmail.com)

Irina O. Petrova, PhD, research assistant (iopetrova17@gmail.com)

Maria G. Korenkova, director of external relations (MGKorenkova@mephi.ru)

Laboratory of Nano-Bioengineering, Moscow Engineering Physics Institute
31 Kashirskoe shosse, 115409 Moscow, Russian Federation
http://www.lnbe.mephi.ru/en

 
 
 
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