Joint project of LNBE and Nanophotonics & Nanomedical Research Group of the Pohang University of Science and Technology, Korea, on Development of New Molecular Imaging Probes Using Single-Domain Antibody–Infrared Quantum Dot Conjugates is about completed

LNBE and the Nanophotonics & Nanomedical Research Group of the Pohang University of Science and Technology (POSTECH), Korea, are successfully completing their joint project. The research activities of the Russian group on this subject have been supported by the Ministry of Education and Science of the Russian Federation under contract no. 14.584.21.0012 in the framework of the Federal Target Program on Research and Development in Priority Fields of the Science and Technology Complex of Russia for 2014–2020; the research carried out by the Korean group has been supported by the Ministry of Education and Science of the Republic of Korea.

The joint research and developments, performed from 30 Jan 2015 to 31 Dec 2016, have yielded diagnostic nanoprobes representing oriented conjugates of semiconductor quantum dots fluorescing in the optical range 700–1700 nm and single-domain antibodies against the HER2 cancer marker. These diagnostic nanoprobes have been used for imaging the tumor marker both in cell cultures and in in vivo animal models.

The project partners have solved the following key tasks:

1. Quantum dots emitting in the infrared spectral range (IR QDs) have been synthesized, and methods for their stabilization in aqueous solutions have been developed.

2. Biocompatible IR QDs with the surfaces modified with different polymers have been fabricated, and their cytotoxicity has been tested in in vitro and in vivo models.

3. Modified recombinant single-domain antibodies (sdAbs) against the HER2 cancer marker have been obtained, and diagnostic nanoprobes in the form of oriented IR QD–sdAb conjugates fluorescing in the human tissue transparency window have been fabricated.

4. The diagnostic nanoprobes have been characterized in terms of sensitivity and specificity and used for imaging of the HER2 tumor marker in cell cultures and in vivo animal models.

Contacts:

Professor Igor Nabiev,
Leading scientist, Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI, head of the Russian group of the joint project, www.lnbe.mephi.ru / www.mephi.ru

Professor Sungjee Kim,
Director, Nanophotonics & Nanomedical Research Group, Pohang University of Science and Technology*, head of the Korean group of the joint project, www.nanotrio.com / www.postech.ac.kr


* POSTECH is ranked #1 in Korea & #8 in Asia in the Times Higher Education Asia Universities Rankings 2016.


 

 
 
 
© 2012 Laboratory of Nano-BioEngineering