A Method for Fabrication of a Regenerable Biosensor Based on a Photonic Crystal–Affine Molecule Complex invented in LNBE

A non-disposable biosensor has been designed in the course of an interdisciplinary project carried out by three of the four research groups of the LNBE. The biosensor, now registered as an invention, is intended for laboratory clinical diagnosis through detecting disease markers in patients' blood.

As in other developments of LNBE for diagnostics, semiconductor quantum dots serve as fluorescent labels in this sensor. One of their advantages over traditionally used labels is a high photostability making it possible to use powerful sources for signal excitation and accumulation. The main part of the sensor is an aggregate of silicon nanowires forming a photonic structure which enhances both the fluorescence of quantum dots and the efficiency of signal harvesting. Antibodies that highly specifically recognize and bind disease biomarkers are covalently linked to the nanowire surface. After each cycle of analysis, the sensor is regenerated in a regenerating solution, and then it can be used for another analysis.

The operating principle and structural characteristics of the biosensor ensure its high sensitivity, which allows it to be used, e.g., to diagnose malignant tumors at the earliest stages, when other diagnostic methods fail. It is expected that, owing to the accurate quantitative assessment of the blood content of biomarkers, the sensor will be suitable not only for diagnosis, but also for monitoring the course of treatment.

Implementation of the LNBE's new invention in clinical practice will make diagnosing a number of socially important diseases faster and less expensive, as well as more accurate and reliable.

The invention has been made in the context of a project supported by the Federal Research and Development Target Program of the Ministry of Education and Science of the Russian Federation (https://xpir.ru/conference2015/theses/14.575.21.0065).


Dmitriy S. Dovzhenko (dovzhenkods@gmail.com)

Laboratory of Nano-Bioengineering,
Moscow Engineering Physics Institute
31 Kashirskoe shosse, 115409 Moscow, Russian Federation


© 2012 Laboratory of Nano-BioEngineering