LNBE presented four reports at the conference summarizing the first stage of the Federal Target Program "Research and Development in Priority Fields of the Science and Technology Complex of Russia for 2014–2020"

LNBE has successfully presented four research projects at the conference that summarized applied research and developments carried out in 2015 in the context of the Federal Target Program "Research and Development in Priority Fields of the Science and Technology Complex of Russia for 2014–2020." The interim results of the projects and expected achievements are published at the Xpir Internet portal.

LNBE presented novel developments that leave far behind the, in terms of effectiveness and efficiency, currently available analogues in burgeoning areas of science and technology such as medical diagnosis based on biomarker detection and nanotechnological measuring instruments.

Three projects deal with early diagnosis of socially significant diseases, which is the principal line of LNBE's research.

The sensor to be designed in the course of the project on Designing of a Prototype Regenerable Nanowire Biosensor for Detecting Serum Markers of Socially Important Diseases, will be far more sensitive than currently available diagnostic systems and will require both less biological material and less time to perform analysis. This will be a non-disposable biosensor compatible with standard laboratory readers. In addition to diagnosis, it will be suitable for controlling the blood content of cancer markers in order to estimate the treatment efficacy.

The planned output of the project on Development of an Analytical System Based on Liquid-State Microarrays for High-Throughput Detection of Cancer Biomarkers is a system of microbeads that carry capture molecules on their surface and are spectrally encoded with uniquely photostable and brightly fluorescing semiconductor nanocrystals (NCs). This will ensure a ten or more times higher sensitivity compared to the existing analogues. The system proposed in the project employs a single source of excitation for fluorescent labels of different colors, which makes the system simpler and less expensive. The multilayered structure of the microbeads prevents distortions of the spectral code caused by resonance energy transfer and emission reabsorption.

The use of NCs fluorescing in the "transparency window" of live tissues (700–1700 nm) is the specific characteristic of nanoprobes that LNBE researchers are developing in the project entitled Development of New Molecular Imaging Probes Using Single-Domain Antibody–Infrared Quantum Dot Conjugates. By using this fluorescence spectral band, one can get rid of the noise signal caused by autofluorescence of live cells. The next-generation biocompatible probes to be designed, in which such NCs are conjugated with single-domain antibodies against the cancer marker HER2, are intended for effective differential laboratory diagnosis and imaging of benign and malignant tumors and metastases.

Originally developed for detection of cancer biomarkers, the new diagnostic systems can be used for diagnosing other socially significant diseases.

The fourth project, Development of an Experimental Method of High-Resolution Multi-Variable Correlation Analysis of the Optical and Morphological Properties in Bulk Nanomaterials, is a response to the ever-growing demand for precise and efficient measuring equipment for analyzing the properties of nanomaterials, whose area of application is rapidly expanding. This project is aimed at designing a measuring instrument with which one could determine the relationship between the optical properties of a nanomaterial and its morphology on the nanoscale in a single area of the material. For this purpose, the novel method of multi-variable scanning probe nanotomography (SPNT) combining scanning probe microscopy, ultramicrotomy, and optical microspectroscopy is used. The technique has a high potential in several branches of science and technology, including photonics, information recording/retrieval, and development of compact laser systems, as well as in biology and biomedicine, where it is applicable, e.g., to pathomorphological studies at the subcellular level.

The implementation of the projects currently underway in LNBE will undoubtedly make a substantial contribution to a number of priority fields of nanotechnology, bioengineering, and medicine.

The Xpir website: https://xpir.ru/ntisearch?format=html&objectType=project&page=1.

Contact:

Alexandra Pavlova, manager of LNBE projects under Federal Target Programs (pav1ova@hotmail.com).

Laboratory of Nano-Bioengineering, Moscow Engineering Physics Institute
31 Kashirskoe shosse, 115409 Moscow, Russian Federation

http://www.lnbe.mephi.ru/en

 
 
 
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