A breakthrough study by Kristina Brazhnik and other researchers of LNBE on quantum-dot-based suspension systems for multiplexed diagnosis of cancer is published by the highly rated Nanomedicine: Nanotechnology, Biology and Medicine

Another highly rated study co-authored by researchers from LNBE; the laboratory headed by Prof. A.Yu. Baryshnikov at the Blokhin Russian Cancer Research Center; University of Reims, France; and Snotra, a Skolkovo resident company, Russia, has been accepted for publication by the Nanomedicine: Nanotechnology, Biology and Medicine, one of the world's leading journals covering biological and biomedical applications of nanomaterials and nanotechnologies (Kristina Brazhnik, Zinaida Sokolova, Maria Baryshnikova, Regina Bilan, Anton Efimov, Igor Nabiev (2015) Quantum dot-based lab-on-a-bead system for multiplexed detection of free and total prostate-specific antigens in clinical human serum samples).

The paper describes the complete cycle of engineering suspension microarrays encoded with fluorescent nanocrystals, including the estimation of their key analytical and diagnostic characteristics with the use of clinical samples. The microarrays are intended for quantitative determination of cancer markers in blood serum and highly effective in vitro diagnosis of malignant tumors, exemplified in this study by prostate cancer.

The operation principle of the multiplexed suspension system is specific visualization and quantitative estimation of each marker included in an immune complex on the surface of optically encoded microparticles by means of flow cytometry. Each marker is assigned a unique optical code of a microbead population, which allows it to be identified and quantitatively estimated in a biological sample. Mixed populations of fluorescent microspheres whose optical codes are assigned to different biomolecules can be used for multiplexed quantitative determination of a specific marker profile in a single sample.

The new generation of diagnostic systems has obvious advantages over their commercially available analogues. The approach described in the paper makes it possible to simultaneously detect multiple cancer markers in a single sample while achieving a higher sensitivity of multiplexed analysis and to reduce the time and cost of immune analysis of several markers. Importantly, the analysis is performed using the standard laboratory equipment.

The published study will serve as the basis for the development of new highly effective and efficient suspension systems based on quantum dots for multiplexed diagnosis of different malignant tumors, first of all, prostate cancer and tumors of the female reproductive system. These diagnostic systems are being developed by LNBE in the context of the Federal Target Program "Research and Developments in the Priority Fields of the Scientific and Technological Complex of Russia for the Years 2014–2020" (contract no. 14.578.21.0054).


Kristina I. Brazhnik, PhD, junior researcher (kris.brazhnik@gmail.com)

Nanomedicine Group,
Laboratory of Nano-Bioengineering,
Moscow Engineering Physics Institute
31 Kashirskoe shosse, 115409 Moscow, Russian Federation


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