Two articles by LNBE researchers are published in the Methods in Molecular Biology, an international series of collections of research methods and protocols cited in the Web of Science and Scopus databases

The two published chapters of the Quantum Dots: Applications in Biology of the Methods in Molecular Biology series describe the methodologies for engineering highly efficient diagnostic nanoprobes based on conjugates of fluorescent nanocrystals (quantum dots) and antibodies.

One of the chapters included in this collection systematizes and describes a unique method for obtaining conjugates of fluorescent nanocrystals with classical full-size antibodies (Brazhnik, K. et al. Advanced Procedure for Oriented Conjugation of Full-Size Antibodies with Quantum Dots). An optimized procedure of partial reduction of full-size antibodies and oriented conjugation of their fragments with the nanocrystal surface makes it possible to obtain specific optical probes based on classical capture biomolecules.

The other chapter describes a method for obtaining highly sensitive compact nanoprobes of a new generation based on conjugates of quantum dots with single-domain antibodies (Brazhnik, K., et al. Oriented Conjugation of Single-Domain Antibodies and Quantum Dots). Recent studies performed by LNBE researchers together with their colleagues from Ireland, the United Kingdom, Belgium, France, and Germany have proved a high efficiency of the nanostructures developed and their usefulness for selective imaging of cancer markers and early diagnosis of cancer.

Numerous studies carried out in LNBE and collaborating foreign research centers have demonstrated the advantages of the compact, highly efficient, and sensitive optical probes based on quantum dots for the diagnosis, monitoring, and imaging of various biological processes and structures. The novel nanoprobes, with their small size and bright fluorescence in a narrow spectral band, allow the target biomarkers to be detected much more reliably compared to their detection using state-of-the-art methods.


Kristina I. Brazhnik, engineer (

Laboratory of Nano-Bioengineering, Moscow Engineering Physics Institute
31 Kashirskoe shosse, 115409 Moscow, Russian Federation

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