Publications from LNBE in Russian journals

Late last year and early this year, Russian journals have published two studies presenting the results of two line of research in LNBE, development of nano–bio hybrid films for efficient utilization of solar energy and engineering of a new generation of systems for early diagnosis of cancer.

In both studies, bioengineered structures containing natural proteins and fluorescent semiconductor nanocrystals (quantum dots, QDs) have been fabricated and analyzed.

The first study, which has been published in the Russian Chemical Reviews, the highest rated Russian journal with an impact factor of 2.299 (Zaitsev, S.Yu., Solovyeva, D.O., and Nabiev, I.R. (2014) Nanobiohybrid structures based on the organized films of photosensitive membrane proteins. Russian Chemical Reviews, 83(1), 38-81), is a review of stable films based on biological chromophores (retinal-containing proteins, reaction centers of photosynthesizing bacteria, light-harvesting complexes, and photosystems I and II) conjugated with metal or metal oxide nanoparticles or QDs. In these structures, QDs, having absorbed sunlight energy, transfer it to chromophores (light-harvesting antennae of photosynthetic systems of various living organisms). The point is, these antennae themselves absorb light in a rather narrow wavelength range, so that the solar energy is used inefficiently. QDs, on the contrary, have a wide absorption spectrum, and they transfer the absorbed energy to biological chromophores nonradiatively (fluorescence or Förster resonance energy transfer, FRET), thereby considerably increasing the efficiency of its utilization in nano–bioengineered devices. Special attention is paid to the obtaining ultrathin, highly organized films at interfaces as the key stage of the design of nano–bio hybrid materials with desired properties for photovoltaics, optoelectronics, and nanophotonics.

The other study (Brazhnik, K.I., Baryshnikova, M.A., Sokolova, Z.A., Nabiev, I.R. (2013) New trends in the study and early diagnosis of cancer with the use of detection systems based on fluorescent nanocrystals. Rossiskii Bioterapevticheskii Zhurnal [Russian Journal of Biotherapy], 12(3), 11–24) deals with the prospects of the use of QDs in multiplexed diagnostic systems (microprobes) that contain antibodies recognizing various cancer biomarkers or, conversely, antigens recognizing autoantibodies circulating in the blood flow of a patient. The use of QD-encoded microbeads allow a specific optical code to be assigned to each microprobe and the amount of a specific biomarker in the analyte to be evaluated. In this case, QDs may themselves determine the fluorescent code of a biomarker, or they may, upon excitation with light, transfer energy to secondary detector luminophores through FRET. The diagnostic systems are multiplexed because they can simultaneously carry capture molecules recognizing different biomarkers in order to form a set of optical codes by using QDs of different sizes fluorescing at different wavelengths. The binding of a probe with its target can be detected then by the fluorescence of the secondary detection tag.

Contacts:

Daria O. Solovyeva, junior researcher (d.solovieva@mail.ru)

Kristina I. Brazhnik, engineer (kris.brazhnik@gmail.com)

Laboratory of Nano-Bioengineering, Moscow Engineering Physics Institute,
31 Kashirskoe shosse, 115409 Moscow, Russian Federation

http://www.lnbe.mephi.ru/en

 
 
 
© 2012 Laboratory of Nano-BioEngineering