Laboratory of Nano-Bioengineering got another patent on invention in medical diagnosis

RF Federal Intellectual Property Agency issued a patent on A Kit for Differential Diagnosis of Diseases, an invention made at the Laboratory of Nano-Bioengineering (LNBE) of NRNU MEPhI and Gamaleya Research Institute of Epidemiology and Microbiology.


The kit consists of populations of polymer microbeads whose surface contains nanoparticles of different chemical nature and with different physical characteristics, nanoparticles of different types being separated from one another with polyelectrolyte layers.

The invention has a prototype, developed in LNBE earlier, where nicrobead surface also carried fluorescent quantum dots and capture molecules. The new kit differs from the prototype in additional surface layers that contain magnetic nanoparticles, which make it possible to separate the microbeads in a magnetic field to obtain purified analytes, and layers of amphoteric polyelectrolytes, which ensure stimulus-sensitive destruction of the microbeads after separation. The hew diagnostic tool allows not only the detection of disease markers or pathogens by means of flow cytometry, but also their detailed analysis using methods that require pure analytes. In addition to improved differential diagnosis, this makes it possible to reduce the cost of analysis by using less expensive instruments and equipment.

The effectiveness of the novel tool for differential diagnosis has been demonstrated in experiments where markers of human immunodeficiency virus and tuberculosis were detected in a sample of biological fluid.

Practical implementation of this invention offers the opportunity of using personalized medicine approaches for planning the cure strategy based on molecular analysis of the disease factors and selection of the optimal treatment.


Maria G. Korenkova, director of external relations (

Pavel M. Sokolov, Ph.D., engineer (

Laboratory of Nano-Bioengineering, Moscow Engineering Physics Institute
31 Kashirskoe shosse, 115409 Moscow, Russian Federation

© 2012 Laboratory of Nano-BioEngineering