A patent on the method for synthesis of polymer microbeads containing imaging agents to be used in bioanalytical studies and medical diagnostics

Researchers of LNBE together with their colleagues from the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences have invented and patented a method for fabrication of polymer microbeads (Generalova, A.N., Sizova, S.V., Manokhina, V.V., Mochalov K.E., Oleinikov, V.A., Nabiev, I.R. A method for the formation of multifunctional nanosystems. RFPatentno. 2532559).

The new method makes it possible to obtain microbeads of a desired size with high biological, chemical, and colloidal stabilities in physiological fluids and unify the procedure for embedding them with imaging agents. Monodisperse suspensions of these beads, conjugated with capture molecules (antibodies, enzymes, etc.), can be used in various bioanalytical studies, in particular, for detecting biomarkers of diseases in biological fluids.

Beads of a given size (from 0.15 to 0.8 μm) are obtained through copolymerization of acrolein and styrene, the resultant core being coated with self-assembling layers of two polyelectrolytes, the positively charged poly-L-lysine and the negatively charged sodium polystyrene sulfonate. The resultant polyelectrolyte complex on the bead surface enhances the colloidal stability of the beads and serves as a substrate for conjugation with imaging agents and capture molecules which provide targeting of the probes.

Various imaging agents can be used with the beads, including fluorescent nanocrystals or quantum dots, organic dyes, and magnetic nanoparticles. Different types of agents can be used simultaneously to obtain hybrid (e.g., fluorescent-and-magnetic) beads. The microbeads fabricated by the new method that contain fluorescent agents luminesce much more intensely than other probes of this type owing to the improved procedure of embedding the tags into the surface layer.

These advantages allow highly sensitive, specific probes to be engineered that could be widely used in basic research and various applied studies.

This is the fourth invention patented since the foundation of our laboratory. To date, researchers of LNBE have registered three more intellectual property objects (two know-hows and one computer software) and have applied for one more patent on an invention.

Contact:

Vladimir A. Oleinikov, PhD, DSc, leader of the Biophysics Group (voleinik@mail.ru)

Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI
(Moscow Engineering Physics Institute)

31 Kashirskoe shosse, 115409 Moscow, Russian Federation

http://www.lnbe.mephi.ru/en

 surface enhances the colloidal stability of the beads and serves as a substrate for conjugation with imaging agents and capture molecules which provide targeting of the probes.

Various imaging agents can be used with the beads, including fluorescent nanocrystals or quantum dots, organic dyes, and magnetic nanoparticles. Different types of agents can be used simultaneously to obtain hybrid (e.g., fluorescent-and-magnetic) beads. The microbeads fabricated by the new method that contain fluorescent agents luminesce much more intensely than other probes of this type owing to the improved procedure of embedding the tags into the surface layer.

These advantages allow highly sensitive, specific probes to be engineered that could be widely used in basic research and various applied studies.

This is the fourth invention patented since the foundation of our laboratory. To date, researchers of LNBE have registered three more intellectual property objects (two know-hows and one computer software) and have applied for one more patent on an invention.

Contact:

Vladimir A. Oleinikov, PhD, DSc, leader of the Biophysics Group (voleinik@mail.ru)

Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI
(Moscow Engineering Physics Institute)

31 Kashirskoe shosse, 115409 Moscow, Russian Federation

http://www.lnbe.mephi.ru/en

 
 
 
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